Efficacy of 3D-Printed Chitosan-Cerium Oxide Dressings Coated With Vancomycin-Loaded Alginate for Chronic Wounds Management

Authors

• Sharareh Shahroudi, K.N.Toosi University of Technology
• Amir Parvinnasab, K.N.Toosi University of Technology
• Erfan Salahinejad, K.N.Toosi University of Technology
• Shaghayegh Abdi, Royan Institute for Stem Cell Biology and Technology
• Sarah Rajabi, Royan Institute for Stem Cell Biology and Technology
• Lobat Tayebi, Marquette UniversityFollow

Document Type

Article

Publication Date

2-2025

Publisher

Elsevier

Source Publication

Carbohydrate Polymers

Source ISSN

0144-8617

Original Item ID

DOI: 10.1016/j.carbpol.2024.123036

Abstract

Multifunctional wound dressings with antibacterial and antioxidant properties hold significant promise for treating chronic wounds; however, achieving a balance of these characteristics while maintaining biocompatibility is challenging. To enhance this balance, this study focuses on the design and development of 3D-printed chitosan-matrix composite scaffolds, which are incorporated with varying amounts of cerium oxide nanoparticles (0, 1, 3, 5, and 7 wt%) and subsequently coated with a vancomycin-loaded alginate layer. The structure, antibiotic drug delivery kinetics, biodegradation, swelling, biocompatibility, antibacterial, antioxidant, and cell migration behaviors of the fabricated dressings were evaluated in-vitro. The findings reveal that all of the formulations demonstrated a robust antibacterial effect against S. aureus bacterial strains in disk diffusion tests. Furthermore, the dressings containing cerium oxide nanoparticles exhibited proper antioxidant capabilities, with over 78.1 % reactive oxygen species (ROS) scavenging efficiency achieved with 7 % cerium oxide nanoparticles. The sample containing 5 % cerium oxide nanoparticles was identified as the optimal formulation, characterized by the most favorable cell biocompatibility, an ROS scavenging ability of over 73.4 %, and the potential to close the wound bed within 24 h. This study highlights that these dressings are promising for managing chronic wounds by preventing infection and oxidative stress in a correct therapeutic sequence.

Comments

Carbohydrate Polymers, Vol. 349, No. B (February 2025). DOI.

Recommended Citation

Shahroudi, Sharareh; Parvinnasab, Amir; Salahinejad, Erfan; Abdi, Shaghayegh; Rajabi, Sarah; and Tayebi, Lobat, "Efficacy of 3D-Printed Chitosan-Cerium Oxide Dressings Coated With Vancomycin-Loaded Alginate for Chronic Wounds Management" (2025). School of Dentistry Faculty Research and Publications. 606.
https://epublications.marquette.edu/dentistry_fac/606