Promoting Angiogenesis/Osteogenesis by a New Copper/Magnesium Hydroxide Hybrid Nanoparticle: In Vitro and In Vivo Investigation

Authors

• Parsa Khalkhali, University of Tehran
• Meisam Omidi, Marquette University
• Daniela S. Masson-Meyers, Marquette University
• Babak Akbari, Univeristy of Tehran
• Mohammad Mehdi Dehghan, University of Tehran
• Hossein Aminianfar, University of Tehran
• Saeed Farzad-Mohajeri, University of Tehran
• Van Mansouri, University of Tehran
• Lobat Tayebi, Marquette UniversityFollow

Document Type

Article

Publication Date

1-2025

Publisher

Wiley

Source Publication

Journal of Biomedical Materials Research. Part A

Source ISSN

1549-3296

Original Item ID

DOI: 10.1002/jbm.a.37855

Abstract

In this study, a new hybrid nanoparticle composed of magnesium hydroxide and copper oxide (Mg(OH)2/CuO) with an optimized ratio of magnesium (Mg) to copper (Cu) was designed and incorporated into a 3D-printed scaffold made of polycaprolactone (PCL) and gelatin. These hybrid nanostructures (MCNs) were prepared using a green, solvent-free method. Their topography, surface morphology, and structural properties were characterized using scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). The fabricated 3D-printed PCL/Gelatin/MCN scaffolds were investigated in vitro and in vivo. Cell viability tests on murine calvarial preosteoblasts (MC3T3-E1) and human umbilical vein endothelial cells (HUVECs) demonstrated that the scaffolds could induce proper cell proliferation. Additionally, the angiogenic and osteogenic properties of the constructs were evaluated using alkaline phosphatase (ALP) activity, osteogenesis-related, and angiogenesis-related gene expression tests. The in vivo study was conducted using a rat calvarial defect model, which confirmed the superior angiogenic and osteogenic properties of the PCL/gelatin/MCN scaffolds compared to PCL/Gelatin and PCL/Gelatin/Mg(OH)2 scaffolds. Overall, the PCL/Gelatin/MCN scaffolds showed promising potential for bone regeneration, particularly for critical-sized defects where proper angiogenesis is essential for tissue reconstruction.

Comments

Accepted version. Journal of Biomedical Materials Research. Part A, Vol. 113, No. 1 (January 2025): e37855. DOI. © 2025 Wiley Periodicals LLC. Used with permission.

Recommended Citation

Khalkhali, Parsa; Omidi, Meisam; Masson-Meyers, Daniela S.; Akbari, Babak; Dehghan, Mohammad Mehdi; Aminianfar, Hossein; Farzad-Mohajeri, Saeed; Mansouri, Van; and Tayebi, Lobat, "Promoting Angiogenesis/Osteogenesis by a New Copper/Magnesium Hydroxide Hybrid Nanoparticle: In Vitro and In Vivo Investigation" (2025). School of Dentistry Faculty Research and Publications. 611.
https://epublications.marquette.edu/dentistry_fac/611