Mesoporous Silica Administration as a New Strategy in the Management of Warfarin Toxicity: An In-Vitro and In-Vivo Study

Authors

• Fatemeh Farjadian, Shiraz University of Medical Sciences
• Fatemeh Parsi, Shiraz University of Medical Sciences
• Reza Heidari, Shiraz University of Medical Sciences
• Khatereh Zarkesh, Hormozgan University of Medical Sciences
• Hamid Reza Mohammadi, Lorestan University of Medical Sciences
• Soliman Mohammadi-Samani, Shiraz University of Medical Sciences
• Lobat Tayebi, Marquette UniversityFollow

Document Type

Article

Publication Date

12-2024

Publisher

Tabriz University of Medical Sciences (TUOMS)

Source Publication

Advanced Pharmaceutical Bulletin

Source ISSN

2228-5881

Original Item ID

DOI: 10.34172/apb.42665

Abstract

Purpose:

Warfarin is one of the most widely used anticoagulants that functions by inhibiting vitamin K epoxide reductase. Warfarin overdose, whether intentional or unintentional, can cause life-threatening bleeding. Here, we present a novel warfarin adsorbent based on mesoporous silica that could serve as an antidote to warfarin toxicity.

Methods:

Amino-functionalized mesoporous silica (MS-NH2) was synthesized based on the co-condensation method through a soft template technique followed by template removal. The prepared structure and functional group were studied by Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD). Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) checked the morphology. The capacity of MS-NH2 in the adsorption of warfarin was evaluated in vitro, at pH=7.4 and pH=1.2. In vivo evaluations were performed in control and warfarin-overdosed animal models. Overdosed animals were treated with MS-NH2 by oral gavage. Biomarkers of organ injury were assessed in animal serum.

Results:

The MS-NH2 was relatively uniform, spherical with defined diameters (400 nm) and porous structure. Synthesized particles had a large surface area (1015 m2 g-1) and mean pore diameter of 2.4 nm which led to considerable adsorption capacity for warfarin 1666 mg/g. In vivo studies revealed that oral administration of MS-NH2 in mice poisoned with warfarin caused a significant difference (P< 0.05) in the International Normalized Ratio (INR) and prothrombin time (PT). Moreover, the warfarin with MS-NH2 group demonstrated a notable decrease in biomarkers associated with tissue damage, such as bilirubin, lactate dehydrogenase (LDH), alanine aminotransferase (ALT), and aspartate aminotransferase (AST).

Conclusion:

The results confirm that MS-NH2 administration can be an effective treatment for warfarin toxicity and could potentially mitigate the adverse effects of warfarin poisoning.

Comments

Advanced Pharmaceutical Bulletin, Vol. 14, No. 4 (December 2024): 883-891. DOI.

Recommended Citation

Farjadian, Fatemeh; Parsi, Fatemeh; Heidari, Reza; Zarkesh, Khatereh; Mohammadi, Hamid Reza; Mohammadi-Samani, Soliman; and Tayebi, Lobat, "Mesoporous Silica Administration as a New Strategy in the Management of Warfarin Toxicity: An In-Vitro and In-Vivo Study" (2024). School of Dentistry Faculty Research and Publications. 616.
https://epublications.marquette.edu/dentistry_fac/616