Study of the Salivary Gland in Sciara Coprophila: Electrophoretic Analysis of Soluble Protein Fractions During the Larval-Pupal Transformation.

Author

Anita Cavagnaro Been

Date of Award

3-1971

Document Type

Dissertation - Restricted

Degree Name

Doctor of Philosophy (PhD)

Department

Biological Sciences

First Advisor

Ellen M. Rasch

Second Advisor

Alapati Krishnakumaran

Third Advisor

Oliver Smith

Fourth Advisor

Robert Rasch

Fifth Advisor

Peter Abramoff

Abstract

The objectives of this study were two-fold. First, the development of a micro-electrophoresis technique to separate and characterize species of soluble proteins from minute tissue samples, and second, the application of this technique to analyze soluble proteins from the salivary gland and other tissues of Sciara coprophila during the larval-pupal transformation. This research provides the groundwork for future studies of the salivary gland, possibly done in conjunction with cytogenic analysis of the gland chromosomes.