"A Study of the Intracellular Corneal Epithelial and Stromal Potential " by James Patrick Fee
 

Date of Award

5-1969

Degree Type

Master's Essay - Restricted

Degree Name

Master of Science (MS)

Department

Biological Sciences

First Advisor

Henry F. Edelhauser

Second Advisor

William J. Stekiel

Third Advisor

James J. Smith

Abstract

A microelectrode technique was used to investigate the potential difference within the epithelial cells of the rabbit cornea. This potential was found to be negative with respect to the reference electrode in Krebs-Hensleit Bicarbonate Ringer’s solution bathing the epithelial cells. As the microelectrode was advanced through the cell layers of the epithelium, the potential became more negative with a maximum value of -60.1 ± 0.8 mv obtained in the basal epithelial cells. On penetration of the stroma, the potential became positive with respect to the basal epithelial potential but remained negative with respect to the reference electrode (-29.5 ± 2.0 mv). The epithelial electrical potential profile of normal corneas was similar to results obtained by Kikkawa (Exp. Eye Res. 3:132-140, 1964).

Comments

A Thesis Submitted to the Faculty of the Graduate School, Marquette University, in Partial Fulfillment of the Requirements for the Degree of Master of Science Department of Physiology, Milwaukee, Wisconsin

Download

Share

COinS