Date of Award

5-1961

Degree Type

Master's Essay - Restricted

Degree Name

Master of Science (MS)

Department

Medical

First Advisor

Joseph F. Kuzma

Second Advisor

Chesley P. Erwin

Third Advisor

Donald Greiff

Abstract

I am stuck by the abrupt change in staining reactions of the epithelial neoplasms of the large bowel. At the transitional zone of all of these neoplasms the normal columnar epithelial cells, which are usually filled with mucin, suddenly change to severely hyperchromatic cells which had lost their mucous quality. The hyperchromaticity involves not only the nuclei but also the cytoplasm of the neoplastic cells as well (See Photo I and II).

This observation, along with dark staining reaction of nuclei of all malignant cells, in general, suggests the idea that this severe affinity can cause death of the cancer cells if we bring the nuclear stain (so-called basic dyes) in contact with the cancer cells.

It was also though that injection of tolerable dose of the basic dyes would not severely affect the normal body cells because the nuclei of the cancer cells which are more basophilic than normal cells, would pick up the injected dyes more rapidly and in greater amount than the normal cells at a given time. Since it was known that the neoplastic cells, in a majority of tumors, have increased nucleic acid and deoxyribose nucleic acid, fulgen reaction on the sections of the same specimen, shown in Photomicrograph I, was tried (Photo III). Strong positive fulgen reaction of the neoplastic cells in these sections encourages the thought that the nuclei of these cells have abundant nucleic acid which may become neutralized by basic dyes and neutralization of these acids would probably seriously interfere with metabolic activities related to cellular division and consequently inhibit the tumor growth. For establishing the anti-cancer effect of the basic dyes, I decided to work on two types of peritoneal cancers in mice using CFW strain of mice and a prominent basic dye from each category of biological stains as follows:

1. Hematoxylin - from the class of Natural dyes

2. Janus Green B - from the group of Nitro and Azo dyes

3. Methylen Blue - from the group of Quinone and Imine dyes

4. Crystal Violet - from the group of Phenyl-Methane dyes

5. Acriflavine - from the group of Xanthene dyes

Comments

A Thesis submitted to the faculty of the Graduate School, Marquette University in Partial Fulfillment of the Requirements for the Degree of Master of Science in Pathology Milwaukee, Wisconsin

Download

Share

COinS