A Murine Model of Latex Allergy-induced Airway Hyperreactivity

Authors

J. C. Thakker, Mount Sinai School of Medicine
J. Q. Xia, Medical College of Wisconsin
David A. Rickaby, Medical College of Wisconsin
Gary S. Krenz, Marquette UniversityFollow
K. J. Kelly, Medical College of Wisconsin
V. P. Kurup, Medical College of Wisconsin
Christopher A. Dawson, Medical College of Wisconsin

Document Type

Article

Language

eng

Format of Original

12 p.

Publication Date

1999

Publisher

Springer

Source Publication

Lung

Source ISSN

0341-2040

Original Item ID

doi: 10.1007/PL00007633

Abstract

Sensitization to latex proteins can cause immediate IgE mast cell-mediated reactions. Health care workers have been found to be particularly at risk because of high exposure. Latex allergy can be produced in mice as demonstrated by IgE and eosinophil responses. Thus the mouse is a potential animal model for studying this disease, but the airway response to latex sensitization in mice has not been evaluated previously. In the present study, we immunized BALB/c mice intranasally with nonammoniated latex proteins. Animals were anesthetized, and lung mechanics were evaluated plethysmographically. Changes in pulmonary conductance (G_L) and compliance (C_dyn) were measured in response to a nonspecific challenge with methacholine or to a direct challenge with intravenous latex antigen. Latex sensitization resulted in elevated levels of IgE and latex-specific IgG₁ as well as interstitial infiltrates consistent with an allergic response. The methacholine dose-response ED₅₀ for G_L was 116.4 μg for the control mice and fell significantly to 20.9 μg for latex-sensitized mice. The ED₅₀ calculated for C_dyn was also significantly lower after latex sensitization. The G_L in latex-sensitized mice challenged with latex antigen fell significantly from a prechallenge value of 1.87 ± 0.41 (S.E.) to 0.198 ± 0.03 ml · s⁻¹· cmH₂O after latex antigen challenge. The results indicate that latex-sensitized mice did exhibit increased airway reactivity in the methacholine challenge test. The latex allergic response in mice is unique in that direct challenge with latex antigen itself also resulted in a significant airway response.

Comments

Lung, Vol. 177, No. 2, (1999): 89-100. DOI.

Shareable Link. Provided by the Springer Nature SharedIt content-sharing initiative.

Recommended Citation

Thakker, J. C.; Xia, J. Q.; Rickaby, David A.; Krenz, Gary S.; Kelly, K. J.; Kurup, V. P.; and Dawson, Christopher A., "A Murine Model of Latex Allergy-induced Airway Hyperreactivity" (1999). Mathematics, Statistics and Computer Science Faculty Research and Publications. 36.
https://epublications.marquette.edu/mscs_fac/36