Genome Resources for Climate-Resilient Cowpea, An Essential Crop for Food Security

Authors

• María Muñoz-Amatriaín, University of California - Riverside
• Hamid Mirebrahim, University of California - Riverside
• Pei Xu, Zhejiang Academy of Agricultural Sciences
• Steve Wanamaker, University of California - Riverside
• MingCheng Luo, University of California - Davis
• Hind Alhakami, University of California - Riverside
• Matthew Alpert, University of California - Riverside
• Ibrahim Atokple, Savanna Agricultural Research Institute
• Benoit J. Batieno, Institut de l'Environnement et de Recherches Agricoles
• Ousmane Boukar, International Institute of Tropical Agriculture
• Serdar Bozdag, Marquette UniversityFollow
• Ndiaga Cisse, Institut Sénégalais de Recherches Agricoles
• Issa Drabo, Institut de l'Environnement et de Recherches Agricoles
• Jeffrey D. Ehlers, University of California - Riverside
• Andrew Farmer, National Center for Genome Resources
• Christian Fatokun, International Institute of Tropical Agriculture
• Yong Q. Gu, USDA-ARS Western Regional Research Center
• Yi-Ning Guo, University of California - Riverside
• Bao-Lam Huynh, University of California - Riverside
• Scott A. Jackson, University of Georgia
• Francis Kusi, Savanna Agricultural Research Institute
• Cynthia T. Lawley, Illumina, Inc.
• Mitchell R. Lucas, University of California - Riverside
• Yaqin Ma, University of California - Riverside
• Michael P. Timko, University of Virginia
• Jiajie Wu, University of California - Davis
• Frank You, University of California - Davis
• Noelle A. Barkley, USDA-ARS Plant Genetic Resources Conservation Unit
• Philip A. Roberts, University of California - Riverside
• Stefano Lonardi, University of California - Riverside
• Timothy J. Close, University of California - Riverside

Document Type

Article

Language

eng

Publication Date

10-2016

Publisher

Wiley

Source Publication

Plant Journal

Source ISSN

0960-7412

Original Item ID

DOI: 10.1111/tpj.13404

Abstract

Cowpea (Vigna unguiculata L. Walp.) is a legume crop that is resilient to hot and drought-prone climates, and a primary source of protein in sub-Saharan Africa and other parts of the developing world. However, genome resources for cowpea have lagged behind most other major crops. Here we describe foundational genome resources and their application to the analysis of germplasm currently in use in West African breeding programs. Resources developed from the African cultivar IT97K-499-35 include a whole-genome shotgun (WGS) assembly, a bacterial artificial chromosome (BAC) physical map, and assembled sequences from 4355 BACs. These resources and WGS sequences of an additional 36 diverse cowpea accessions supported the development of a genotyping assay for 51 128 SNPs, which was then applied to five bi-parental RIL populations to produce a consensus genetic map containing 37 372 SNPs. This genetic map enabled the anchoring of 100 Mb of WGS and 420 Mb of BAC sequences, an exploration of genetic diversity along each linkage group, and clarification of macrosynteny between cowpea and common bean. The SNP assay enabled a diversity analysis of materials from West African breeding programs. Two major subpopulations exist within those materials, one of which has significant parentage from South and East Africa and more diversity. There are genomic regions of high differentiation between subpopulations, one of which coincides with a cluster of nodulin genes. The new resources and knowledge help to define goals and accelerate the breeding of improved varieties to address food security issues related to limited-input small-holder farming and climate stress.

Comments

Published version. Plant Journal, Vol. 89, No. 5 (October 2016): 1042-1054. DOI. © 2016 The Authors. Used with permission. Used with permission.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License

Recommended Citation

Muñoz-Amatriaín, María; Mirebrahim, Hamid; Xu, Pei; Wanamaker, Steve; Luo, MingCheng; Alhakami, Hind; Alpert, Matthew; Atokple, Ibrahim; Batieno, Benoit J.; Boukar, Ousmane; Bozdag, Serdar; Cisse, Ndiaga; Drabo, Issa; Ehlers, Jeffrey D.; Farmer, Andrew; Fatokun, Christian; Gu, Yong Q.; Guo, Yi-Ning; Huynh, Bao-Lam; Jackson, Scott A.; Kusi, Francis; Lawley, Cynthia T.; Lucas, Mitchell R.; Ma, Yaqin; Timko, Michael P.; Wu, Jiajie; You, Frank; Barkley, Noelle A.; Roberts, Philip A.; Lonardi, Stefano; and Close, Timothy J., "Genome Resources for Climate-Resilient Cowpea, An Essential Crop for Food Security" (2016). Mathematics, Statistics and Computer Science Faculty Research and Publications. 466.
https://epublications.marquette.edu/mscs_fac/466