Cell-Mediated Immunity and Rat Pulmonary Late Allergic Responses [abstract]

Document Type

Article

Language

eng

Format of Original

1 p.

Publication Date

1-1993

Publisher

Elsevier

Source Publication

Journal of Allergy and Clinical Immunology

Source ISSN

0091-6749

Abstract

We have previously reported that the formation of antigen-specific IgE antibody in rats is associated with the induction of immediate but no late changes (airway inflammation and increased resistance) following pulmonary antigen challenge. Rats sensitized to dinitrophenol (DNP)-ovalbumin (OA) develop IgE and IgG anti-DNP antibodies and isolated immediate but no late responses when challenged with DNP-BSA; in contrast, only IgG anti-OA is simultaneously produced and OA challenge results in mild immediate and substantial late pulmonary reactions. To determine if cell-mediated immune responses may also be relevant to these observed differences in responses, we measured in vitro lymphocyte proliferative responses to DNP-BSA, OA, and concanavalin A (Con A) in both sensitized and control rats. Splenic and blood lymphocytes were cultured with varying concentrations of antigen (0.5, 5, 50, 500 µg/ml), pulsed with 1 % tritiated thymidine on day 3 or 7, and counted 8 hours later. Cells obtained from sensitized rats developed dose-dependent response to OA (P

Comments

Journal of Allergy and Clinical Immunology, Vol. 91, No. 1, Part 2 (January 1993): 238. DOI.

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