Cell-Mediated Immunity and Rat Pulmonary Late Allergic Responses [abstract]
Format of Original
Journal of Allergy and Clinical Immunology
Original Item ID
Shelves: RC583 .J Memorial Periodicals
We have previously reported that the formation of antigen-specific IgE antibody in rats is associated with the induction of immediate but no late changes (airway inflammation and increased resistance) following pulmonary antigen challenge. Rats sensitized to dinitrophenol (DNP)-ovalbumin (OA) develop IgE and IgG anti-DNP antibodies and isolated immediate but no late responses when challenged with DNP-BSA; in contrast, only IgG anti-OA is simultaneously produced and OA challenge results in mild immediate and substantial late pulmonary reactions. To determine if cell-mediated immune responses may also be relevant to these observed differences in responses, we measured in vitro lymphocyte proliferative responses to DNP-BSA, OA, and concanavalin A (Con A) in both sensitized and control rats. Splenic and blood lymphocytes were cultured with varying concentrations of antigen (0.5, 5, 50, 500 µg/ml), pulsed with 1 % tritiated thymidine on day 3 or 7, and counted 8 hours later. Cells obtained from sensitized rats developed dose-dependent response to OA (P<.0001) but no significant response to DNP-BSA. Cells from control rats did not response to either OA or DNP-BSA. DNP-BSA did not decrease OA-induced responses, indicating no toxic effect on the prolierative response. Since the pattern of in vitro lymphocyte proliferative response to antigen parallels the presence or absence of a late reaction in vivo with the same antigen, our results indicate that sensitized lymphocytes contribute to the cellular and physiologic alterations that occur during the late reaction in this species.