Effects on Breathing of Agonists to μ-opioid or GABA_A Receptors Dialyzed into the Ventral Respiratory Column of Awake and Sleeping Goats

Authors

Thomas M. Langer III, Medical College of WisconsinFollow
Suzanne E. Neumueller, Medical College of WisconsinFollow
Emma Crumley, Medical College of Wisconsin
Nicholas J. Burgraff, Medical College of Wisconsin
Sawan Talwar, Medical College of Wisconsin
Matthew R. Hodges, Medical College of Wisconsin
Lawrence Pan, Marquette UniversityFollow
Hubert V. Forster, Medical College of Wisconsin

Document Type

Article

Language

eng

Publication Date

5-2017

Publisher

Elsevier

Source Publication

Respiratory Physiology & Neurobiology

Source ISSN

1569-9048

Abstract

Pulmonary ventilation (V̇_I) in awake and sleeping goats does not change when antagonists to several excitatory G protein-coupled receptors are dialyzed unilaterally into the ventral respiratory column (VRC). Concomitant changes in excitatory neuromodulators in the effluent mock cerebral spinal fluid (mCSF) suggest neuromodulatory compensation. Herein, we studied neuromodulatory compensation during dialysis of agonists to inhibitory G protein-coupled or ionotropic receptors into the VRC. Microtubules were implanted into the VRC of goats for dialysis of mCSF mixed with agonists to either μ-opioid (DAMGO) or GABA_A (muscimol) receptors. We found: (1) V̇_I decreased during unilateral but increased during bilateral dialysis of DAMGO, (2) dialyses of DAMGO destabilized breathing, (3) unilateral dialysis of muscimol increased V̇_I, and (4) dialysis of DAMGO decreased GABA in the effluent mCSF. We conclude: (1) neuromodulatory compensation can occur during altered inhibitory neuromodulator receptor activity, and (2) the mechanism of compensation differs between G protein-coupled excitatory and inhibitory receptors and between G protein-coupled and inotropic inhibitory receptors.

Comments

Accepted version. Respiratory Physiology & Neurobiology, Vol. 239 (May 2017): 10-25. DOI. © 2018 Elsevier B.V. Used with permission.

Recommended Citation

Langer, Thomas M. III; Neumueller, Suzanne E.; Crumley, Emma; Burgraff, Nicholas J.; Talwar, Sawan; Hodges, Matthew R.; Pan, Lawrence; and Forster, Hubert V., "Effects on Breathing of Agonists to μ-opioid or GABA_A Receptors Dialyzed into the Ventral Respiratory Column of Awake and Sleeping Goats" (2017). Physical Therapy Faculty Research and Publications. 147.
https://epublications.marquette.edu/phys_therapy_fac/147