Document Type

Article

Language

eng

Publication Date

1-1-2015

Publisher

Elsevier

Source Publication

Respiratory Physiology & Neurobiology

Source ISSN

1569-9048

Original Item ID

DOI: 10.1016/j.resp.2014.09.010

Abstract

Reverse dialysis of the muscarinic receptor antagonist, atropine (ATR, 50 mM), into the pre-Bötzinger Complex region of the ventral respiratory column (VRC) of awake and sleeping goats increases breathing frequency and serotonin (5-HT), substance P (SP), glycine, and GABA concentrations in the effluent dialysate. Herein, we report data from goats in which we reverse dialyzed 5 mM ATR or specific antagonists of M2 or M3 muscarinic receptors into the VRC. The effects on frequency of all three antagonists were not significantly different from time control studies. 5 mM ATR and the M3 antagonist increased SP sevenfold less than 50 mM ATR. The antagonists had no effect on 5-HT, glycine, and/or GABA, suggesting that the increases in glycine and GABA with 50 mM ATR were secondary to the larger increases in 5-HT and/or SP. These data are suggestive of neuromodulator interdependence, whereby attenuation of one neuromodulator is compensated for by local changes in other neuromodulators to stabilize breathing.

Comments

Accepted version. Respiratory Physiology & Neurobiology, Vol. 205 (January 1, 2015): 7-15. DOI. © 2015 Elsevier. Used with permission.

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