argE-Encoded N-Acetyl-l-Ornithine Deacetylase from Escherichia coli Contains a Dinuclear Metalloactive Site
Format of Original
American Chemical Society
Journal of the American Chemical Society
Original Item ID
The catalytic and structural properties of the argE-encoded N-acetyl-l-ornithine deacetylase (ArgE) from Escherichia coli were investigated. On the basis of kinetic and ITC (isothermal titration calorimetry) data, Zn(II) binds to ArgE with Kd values that differ by ∼20 times. Moreover, ArgE exhibits ∼90% of its full catalytic activity upon addition of one metal ion. Therefore, ArgE behaves similarly to the aminopeptidase from Aeromonas proteolytica (AAP) in that one metal ion is the catalytic metal ion while the second likely plays a structural role. The N-acetyl-l-ornithine (NAO) deacetylase activity of ArgE showed a linear temperature dependence from 20 to 45 °C, indicating that the rate-limiting step does not change over this temperature range. The activation energy for NAO hydrolysis by ArgE was 25.6 kJ/mol when loaded with Zn(II) and 34.3 kJ/mol when loaded with Co(II). Electronic absorption and EPR (electron paramagnetic resonance) spectra of [Co·(ArgE)] and [CoCo(ArgE)] indicate that both divalent metal binding sites are five coordinate. In addition, EPR data show clear evidence of spin−spin coupling between the Co(II) ions in the active site but only after addition of a second equivalent of Co(II). Combination of these data provides the first physical evidence that the ArgE from E. coli contains a dinuclear Zn(II) active site, similar to AAP and the carboxypeptidase G2 from Pseudomonas sp. strain RS-16 (CPG2).
McGregor, Wade C.; Swierczek, Sabina I.; Bennett, Brian; and Holz, Richard C., "argE-Encoded N-Acetyl-l-Ornithine Deacetylase from Escherichia coli Contains a Dinuclear Metalloactive Site" (2005). Physics Faculty Research and Publications. 27.
Accepted version. Journal of the American Chemical Society, Vol. 127, No. 40 (October 2005): 14100-14107. DOI. © 2005 American Chemical Society. Used with permission.
Brian Bennett was affiliated with Medical College of Wisconsin at the time of publication.
Richard Holz was affiliated with Utah State University at the time of publication.