Structural Investigation of the Molybdenum Site of the Periplasmic Nitrate Reductase from Thiosphaera pantotropha by X-ray Absorption Spectroscopy
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Portland Press Limited
The molybdenum centre of the periplasmic respiratory nitrate reductase from the denitrifying bacterium Thiosphaera pantotropha has been probed using molybdenum K-edge X-ray absorption spectroscopy. The optimum fit of the Mo(VI) EXAFS suggests two O, three –S– and either a fourth –S– or an –O–/–N– as molybdenum ligands in the ferricyanide-oxidized enzyme. Three of the –S– ligands are proposed to be the two sulphur atoms of the molybdopterin dithiolene group and Cys-181. Comparison of the EXAFS of the ferricyanide-oxidized enzyme with that of a nitrate-treated sample containing 30% Mo(V) suggests that the Mo(VI) Mo(V) reduction is accompanied by conversion of one O to –O–. The best fit to the Mo(IV) EXAFS of dithionite-reduced enzyme was obtained using one O, one –O– and four –S–/–Cl ligands. The periplasmic nitrate reductase molybdenum co-ordination environment in both the Mo(VI) and Mo(IV) oxidation states is distinct from that found in the membrane-bound respiratory nitrate reductase.
Bennett, Brian; Charnock, John M.; Sears, Heather J.; Berks, Ben C.; Thomson, Andrew J.; Ferguson, Stuart J.; Garner, C. David; and Richardson, David J., "Structural Investigation of the Molybdenum Site of the Periplasmic Nitrate Reductase from Thiosphaera pantotropha by X-ray Absorption Spectroscopy" (1996). Physics Faculty Research and Publications. 98.