Date of Award
Summer 1957
Document Type
Thesis - Restricted
Degree Name
Master of Science (MS)
Department
Chemistry
First Advisor
Karl, Hermann
Second Advisor
Kittsley, Scott L.
Third Advisor
Bournique, Raymond A.
Abstract
It has been known for a long time (9) that a 3-pyridiool solution produces a red colored solution with ferric chloride. But there have been no known attempts to determine whether this qualitative test could be the basis for a spectrophotometric analysis. An attempt was made to develop a colorimetric method, to determine quantitatively 3-pyridinol, by means of the blue solution developed with Folin-Denis reagent (12). But this method failed because the reaction was a time reaction. The blue color of the solution rapidly became turbid and deposited a white precipitate. A coupling reaction of 3-pyridinol with diazotized p-sulfanilie acid results in an orange colored solution. A spectrophotometric method using this solution (12) was developed. However the accuracy of this method extended only in the region of 0.02 to 0.08 mg. of 3-pyridinol. An accurate and rapid quantitative method is needed to determine 3-pyridinol. This compound is a pharmaceutical intermediate, and a quantitative analysis is needed for quality control work, an important phase of the pharmaceutical industry. The subject of this thesis is the attempt to determine whether the red solution, obtained through the action of ferric chloride on a 3- pyridinol solution, obeys Beer's law. The effects of some inorganic salts on the reaction and Beer's law are also investigated.
Recommended Citation
Janicki, Casimir A., "The 3-Pyridinol-Ferric Chloride Reaction and Beer's Law" (1957). Master's Theses (1922-2009) Access restricted to Marquette Campus. 2566.
https://epublications.marquette.edu/theses/2566