Date of Award

Summer 2000

Document Type

Thesis - Restricted

Degree Name

Master of Science (MS)

Department

Chemistry

Abstract

The generation of ammonia from nitrite is a very important stage in the supply of nitrogen to the majority of the plants in the world. The reduction reaction of nitrite to ammonia is catalyzed by a heme-containing enzyme, usually termed assimilatory nitrite reductase, which can be mimicked by iron porphyrin complexes. In the past two decades the investigation of iron porphyrins has been the subject of intense effort, and considerable data have been accumulated in this field. However, there are still many questions that need be addressed regarding the mechanism for the reduction of nitrite as well as the structure of intermediates during the reactions. It is the aim of this work to solve some of the puzzles by the use of IR spectroelectrochemical technique and surface chemically modifying methods. Chapter 1 reviews the recent progress in the study of iron porphyrin complexes by electrochemical and IR spectroelectrochemical methods. Chapter 2 provides the information about the experimental conditions such as chemicals and instrumental methods. Chapter 3 describes the results and discussion regarding the IR spectroelectrochemistry of iron porphyrin nitrosyl, as well as a novel surface incorporation method to fabricate an iron porphyrin film electrode.

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