Date of Award

Spring 1985

Document Type

Thesis - Restricted

Degree Name

Master of Science (MS)

Department

Biology

First Advisor

Scheffel, James W.

Second Advisor

Abramoff, Peter

Third Advisor

Fredricks, Walter

Abstract

Human T lymphocytes display membrane structures reactive with sheep erythrocytes which cause the formation of non-immune rosettes in vitro. This sheep erythrocyte receptor (E receptor) has been found only on thymus-derived lymphocytes and has been used as a definitive marker for the human T cell. vii Several monoclonal antibodies have been produced which bind specifically to the E receptor causing inhibition of rosette formation and/or modulation of normal T cell function in immunological in vitro assays. All of these anti-E receptor monoclonal antibodies have been shown to precipitate a glycoprotein of 45,000-50,000 daltons from the T cell membrane. I have developed three additional monoclonal antibodies which appear to be reactive with the E receptor. All antibodies (2A5, 4H3, and 6H10) were initially screened utilizing a plate rosetting assay. Subsequent screenings were done with human T and B cell lines as well as normal peripheral blood lymphocytes to determine the reactivity of these antibodies. The ability of these various cells to form rosettes or to be bound by commercial anti-E receptor monoclonal antibodies correlated well with their binding ability to 4H3, 2A5, or 6H10 antibodies. This binding was shown by indirect immunofluorescence. Furthermore, all three of these monoclonal antibodies were reactive with a protein of approximately 50,000 daltons isolated from the T cell membrane.

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