Date of Award
Spring 1962
Document Type
Thesis - Restricted
Degree Name
Master of Science (MS)
Department
Nursing
First Advisor
Laskowski, Michael
Second Advisor
Peanasky, Robert J.
Third Advisor
Haymaker, C. R.
Abstract
Various leguminosae have been found to contain compounds which inhibit the proteolytic action of trypsin. In 1949, Tauber et al. (30) reported that protein crystals obtained from lima beans had the power to inhibit the proteolytic action of trypsin. A few years later, however, Fraenkel-Conrat et al. (6) by modifying Tauber's recrystallization method found that the mother liquor contained a high trypsin inhibiting activity. The crystals were found to be devoid of activity after two recrystallizations. Fraenkel-Conrat's preparation was two and one half times as powerful as Tauber's inhibitor. Recently, Jirgensons et al. (12) found that Fraenkel-Conrat's preparation can be further subdivided by DEAE-cellulose column chromatography into at least six fractions, five of which possessed almost identical activities. This thesis was undertaken to attempt the use of chromatography as a simpler method of separating the active lima bean trypsin inhibitor from Tauber's inactive protein crystals and to define conditions for separation of fractions observed by Jirgensons.
Recommended Citation
Carrillo, Angel Luis, "The Partial Purification of Trypsin Inhibitor from Lima Bean" (1962). Master's Theses (1922-2009) Access restricted to Marquette Campus. 3220.
https://epublications.marquette.edu/theses/3220