Date of Award
Spring 1964
Document Type
Thesis - Restricted
Degree Name
Master of Science (MS)
Department
Nursing
First Advisor
Laskowski, Michael
Second Advisor
Pearasky, Robert J.
Third Advisor
Haymaker, C. R.
Abstract
During the last decade, the most spectacular advances in biochemistry have been made in the study of enzyme systems involved in the synthesis of nucleic acids. In a somewhat less spectacular fashion however, studies have been continued on the purification and characterization of enzymes called nucleases which degrade ribonucleic acid (RNA) or deoxyribonucleic acid (DNA). At present several nucleases (15,23) have been highly purified, and others sufficiently purified to permit characterization of their mode of action. Such nucleases can serve as tools in the study of the structure of nucleic acid and characterization of products and precursors involved in nucleic acid metabolism. In addition to this analytical value, nucleases of known specificities are useful in the preparation of substrates of known chemical composition and properties, which are used in the study of specificities of other enzymes. Since it is beneficial to have a variety of such tools, the present project was undertaken. It was hoped that a nuclease, known to be present in germinating barley, could be purified and its mode of action defined, to implement further studies of nucleic acid chemistry.
Recommended Citation
Schmid, Carol M., "Some Properties of a Crude Preparation of Deoxyribonuclease from Barley" (1964). Master's Theses (1922-2009) Access restricted to Marquette Campus. 3727.
https://epublications.marquette.edu/theses/3727