Date of Award
Spring 1963
Document Type
Thesis - Restricted
Degree Name
Master of Science (MS)
Department
Nursing
First Advisor
Laskowski, Michael
Second Advisor
Roll, Paul M.
Third Advisor
Haymaker, C. R.
Abstract
The first mention of lima bean as a source of trypsin inhibitor was made by Borchers, Ackerson and Kimmet (1). In 1949 Tauber et al. (35) crystallized a protein from lima bean possessing strong trypsin inhibiting activity. By a slight modification of Tauber's process Fraenkel-Conrat et al. (6) separated the active material from the inactive crystals. Jirgenson et al. (15) subdivided the active fraction from lima bean into six nonhomogeneous fractions, five of which possessed almost identical activity. Investigations in this laboratory during the past two years by Angel Carillo indicated the presence of at least three different substances with trypsin inhibiting activity in lima beans. This thesis was undertaken to obtain these distinct trypsin inhibitors in chromatographically pure and crystalline fora. Early in 1963, when this work was well advanced, Jones, Moore and Stein (16) isolated four distinct chromatographically pure substances with trypsin inhibiting activity from lima beans. Essentially very similar results were obtained in this thesis.
Recommended Citation
Matthai, Susan, "The Chromatographic Separation of Trypsin Inhibitors from Lima Beans" (1963). Master's Theses (1922-2009) Access restricted to Marquette Campus. 3941.
https://epublications.marquette.edu/theses/3941