Date of Award

1974

Document Type

Thesis - Restricted

Degree Name

Master of Science (MS)

Department

Biomedical Engineering

First Advisor

Sances, Anthony

Second Advisor

Richardson, Bobbie

Third Advisor

Smith, James

Abstract

The accurate and precise measurement of ph, or hydrogen ion activity, in biological systems in quite complex. Chemical indicators are not usually satisfactory due to inadequate sensitivity, difficulty of detection, or interaction with the biological materials. Electrometric systems of several types are widely used, but cross comparisons are difficult and fixation of a valid reference state is perplexing. Protein interaction with electrode response, errors due to other anions and cations, the effects of colloids and suspensions, and variation of liquid junction potentials of reference electrodes are some of the sources of variability encountered in biological systems. This manuscript examines the suitability of the iridium oxide ph electrode for use in biological fluids and compares its performance with various other methods.

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