Date of Award

5-1958

Document Type

Thesis - Restricted

Degree Name

Master of Science (MS)

Department

Medical

First Advisor

Andrew A. Pandazi

Second Advisor

James J. Smith

Abstract

The discovery of the principles of polarography by Heyrovsky in 1922 may have given science a new tool for the study of some biological processes. Amperometric analysis can be used with a high degree of precision in inorganic solutions; however, in biological fluids such as plasma and whole blood, many complicating factors arise.

Extensive work has been done in the past ten years to make this tool usable for accurate, continuous, and direct estimation of oxygen tension in body fluids; up to this time, however, no single reliable quantitative and practical method is available.

The first objective of this investigation was .to develop a suitable polarograph and a modus operandi which could be easily applied to estimation of oxygen tension in biological fluids. The second objective was to study its characteristics in vitro in blood and plasma and ultimately to apply it to an in vivo preparation.

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