Date of Award
12-1972
Document Type
Thesis - Restricted
Degree Name
Master of Science (MS)
Department
Biological Sciences
First Advisor
Peter Abramoff
Abstract
An anti-bovine serum albumin skin-sensitizing antibody (SSA) is produced in the pigeon which has characteristics of both Type I and Type II homocytotropic antibodies. Antibody production was obtained with or without the use of complete Freund's adjuvant and was recalled after multiple injections of antigen. Pigeon SSA had an optimum passive cutaneous anaphylaxis (PCA) latent period of 72 hours, but was active as early as 4 hours, and as late as 14 days, after passive intradermal transfer. Pigeon SSA produced PCA only in pigeons and doves, a closely related species. Heating at 56° C for 4 hour destroyed the PCA activity of low titered antisera, but did not significantly effect high titered antisera. Analysis by starch block electrophoresis and Sephandex gel chromatography revealed that pigeon SSA was a 7S, yl-globulin which, in some cases, could be correlated with the hemagglutinating activity of the serum.
Recommended Citation
Guttman, Ralph M., "Pigeon Homocytotropic Antibody" (1972). Master's Theses (1922-2009) Access restricted to Marquette Campus. 5610.
https://epublications.marquette.edu/theses/5610