Document Type

Article

Language

eng

Format of Original

6 p.

Publication Date

4-1988

Publisher

American Society for Microbiology

Source Publication

Molecular and Cellular Biology

Source ISSN

0270-7306

Original Item ID

doi: 10.1128/MCB.8.4.1664

Abstract

The extrachromosomal rRNA genes (rDNA) of Tetrahymena thermophila contain 0.4% N6-methyladenine. C3 strain rDNA was isolated, hypermethylated in vitro, and microinjected into B strain host cells. Clonal cell lines were established, and transformants were selected on the basis of resistance to paromomycin, conferred by the injected rDNA. The effects of methylation by three enzymes which methylate the sequence 5'-NAT-3', the dam, EcoRI, and ClaI methylases, were tested. Hypermethylation of the injected rDNA had no effect on transformation efficiency relative to mock-methylated controls. The injected C3 strain rDNA efficiently replaced host rDNA as the major constituent of the population of rDNA molecules. Hypermethylation of the injected DNA was not maintained through 20 to 25 cell generations.

Comments

Published version. Molecular and Cellular Biology, Vol. 8, No. 4 (April 1988): 1664-1669. Permalink. © American Society for Microbiology 1988. Used with permission.

Kathleen Karrer was affiliated with Brandeis University at the time of publication.

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