Cosmid Cloning of Five Zymomonas trp Genes by Complementation of Escherichia coli and Pseudomonas putida trp Mutants
Document Type
Article
Language
eng
Format of Original
6 p.
Publication Date
7-1988
Publisher
American Society for Microbiology
Source Publication
Journal of Bacteriology
Source ISSN
0021-9193
Original Item ID
DOI: 10.1128/jb.170.7.3158-3163.1988
Abstract
A library of Zymomonas mobilis genomic DNA was constructed in the broad-host-range cosmid pLAFR1. The library was mobilized into a variety of Escherichia coli and Pseudomonas putida trp mutants by using the helper plasmid pRK2013. Five Z. mobilis trp genes were identified by the ability to complement the trp mutants. The trpF, trpB, and trpA genes were on one cosmid, while the trpD and trpC genes were on two separate cosmids. The organization of the Z. mobilis trp genes seems to be similar to the organization found in Rhizobium spp., Acinetobacter calcoaceticus, and Pseudomonas acidovorans. The trpF, trpB, and trpA genes appeared to be linked, but they were not closely associated with trpD or trpC genes.
Recommended Citation
Eddy, Christina Kay; Smith, Oliver H.; and Noel, K. Dale, "Cosmid Cloning of Five Zymomonas trp Genes by Complementation of Escherichia coli and Pseudomonas putida trp Mutants" (1988). Biological Sciences Faculty Research and Publications. 400.
https://epublications.marquette.edu/bio_fac/400
Comments
Published version. Journal of Bacteriology, Vol. 170, No. 7 (July 1988): 3158-3163.DOI. © 1988 American Society for Microbiology. Used with permission.