Isolation of Auxotrophs and Analysis of Regulation of Tryptophan Biosynthesis in Zymomonas mobilis

Authors

Christina Kay Eddy, Marquette UniversityFollow
K. Dale Noel, Marquette UniversityFollow
Oliver H. Smith, Marquette University

Document Type

Article

Language

eng

Publication Date

4-1988

Publisher

Springer

Source Publication

Archives of Microbiology

Source ISSN

0302-8933

Abstract

Tryptophan auxotrophs were isolated and used to analyze the regulation of tryptophan biosynthesis in Zymomonas mobilis. Twelve tryptophan auxotrophs were cassified as trp E, Bor A based on accumulation of, or growth on, indole and anthranilic acid. Trp B mutants were found to accumulate indole when grown on limiting, but not on excess tryptophan, suggesting that tryptophan plays a role in regulating its biosynthesis. Tryptophan synthase and indoleglycerol phosphate synthase specific activities were measured in the wild-type strain and two trp mutants grown in limiting or excess tryptophan. Neither activity was repressed by exogenous tryptophan.

Comments

Archives of Microbiology, Vol. 149, No. 6 (April 1988): 561-564. DOI.

Recommended Citation

Eddy, Christina Kay; Noel, K. Dale; and Smith, Oliver H., "Isolation of Auxotrophs and Analysis of Regulation of Tryptophan Biosynthesis in Zymomonas mobilis" (1988). Biological Sciences Faculty Research and Publications. 583.
https://epublications.marquette.edu/bio_fac/583