Document Type

Article

Language

eng

Format of Original

17 p.

Publication Date

2002

Publisher

Oxford University Press

Source Publication

Nucleic Acids Research

Source ISSN

0305-1048

Original Item ID

doi: 10.1093/nar/30.6.1364

Abstract

There are about 50 copies of each chromosome in the somatic macronucleus of the ciliated protozoan Tetrahymena. Approximately 0.8% of the adenine residues in the macronuclear DNA of Tetrahymena are methylated to N6-methyladenine. The degree of methylation varies between sites from a very low percentage to >90%. In this study a correlation was found between nucleosome positioning and DNA methylation. Eight GATC sites with different levels of methylation were examined. There was a direct correlation between the degree of methylation and proximity to linker DNA at these sites. Although methylation occurs preferentially in linker DNA, the patterns and extent of methylation in a histone H1 knockout strain were virtually indistinguishable from those in wild-type cells.

Comments

Published version. Nucleic Acids Research, Vol. 30, No. 6 (2002): 1354-1370. DOI. © Oxford University Press 2002. Used with permission.

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