Methylation of Adenine in the Nuclear DNA of "Tetrahymena" is Internucleosomal and Independent of Histone H1

Authors

Kathleen M. Karrer, Marquette UniversityFollow
Teresa Ann Van Nuland, Marquette University

Document Type

Article

Language

eng

Format of Original

17 p.

Publication Date

2002

Publisher

Oxford University Press

Source Publication

Nucleic Acids Research

Source ISSN

0305-1048

Original Item ID

doi: 10.1093/nar/30.6.1364

Abstract

There are about 50 copies of each chromosome in the somatic macronucleus of the ciliated protozoan Tetrahymena. Approximately 0.8% of the adenine residues in the macronuclear DNA of Tetrahymena are methylated to N⁶-methyladenine. The degree of methylation varies between sites from a very low percentage to >90%. In this study a correlation was found between nucleosome positioning and DNA methylation. Eight GATC sites with different levels of methylation were examined. There was a direct correlation between the degree of methylation and proximity to linker DNA at these sites. Although methylation occurs preferentially in linker DNA, the patterns and extent of methylation in a histone H1 knockout strain were virtually indistinguishable from those in wild-type cells.

Comments

Published version. Nucleic Acids Research, Vol. 30, No. 6 (2002): 1354-1370. DOI. © 2002 Oxford University Press. Used with permission.

Recommended Citation

Karrer, Kathleen M. and Van Nuland, Teresa Ann, "Methylation of Adenine in the Nuclear DNA of "Tetrahymena" is Internucleosomal and Independent of Histone H1" (2002). Biological Sciences Faculty Research and Publications. 62.
https://epublications.marquette.edu/bio_fac/62