Identification of Diverse Bacteriophages Associated with Bees and Hoverflies

Document Type

Article

Publication Date

1-30-2025

Publisher

MDPI

Source Publication

Viruses

Source ISSN

1999-4915

Original Item ID

DOI: 10.3390/v17020201

Abstract

Bacteriophages are the most numerous, ubiquitous, and diverse biological entities on the planet. Prior studies have identified bacteriophages associated with pathogenic and commensal microbiota of honeybees. In this study we expand on what is known about bacteriophages from the lineages Caudoviricetes, Inoviridae, and Microviridae, which are associated with honeybees (Apidae, Apis mellifera), solitary bees of the genus Nomia (Halictidae, Nomia), and hoverflies (Syrphidae). The complete genomes of seven caudoviruses, seven inoviruses, and 288 microviruses were assembled from honeybees (n = 286) and hoverflies in Arizona (n = 2). We used bacterial host predictive software and sequence read mapping programs to infer the commensal and transient bacterial hosts of pollinating insects. Lastly, this study explores the phylogenetic relationships of microviruses sampled from bees, opportunistically sampled pollinating insects such as hoverflies, and blackflies.

Comments

Viruses, Vol. 17, No. 2 (January 30, 2025). DOI.

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