The lim-7p::ced-1::GFP Transgene From the MD701 Strain Increases Embryonic Lethality in c. elegans

Authors

• Nina M. Zampetti, Marquette University
• Kristin A. Quaglia, Marquette University
• Lisa N. Petrella, Marquette UniversityFollow

Document Type

Article

Publication Date

2025

Publisher

California Institute of Technology

Source Publication

microPublication Biology

Source ISSN

2578-9430

Original Item ID

DOI: 10.17912/micropub.biology.001420

Abstract

The lim-7p::ced-1::GFP transgene has been widely used for evaluating germline apoptosis in C. elegans. Here we observed an increase in embryonic lethality in the MD701 strain that contains the lim-7p::ced-1::GFP transgene and a strain that outcrossed the lim-7p::ced-1::GFP transgene into the N2 wild-type strain. While the outcrossed strain had a significantly lower level of embryonic lethality than MD701, it still showed significantly higher embryonic lethality than wild type. Our results suggest that the presence of the lim-7p::ced-1::GFP transgene significantly increases embryonic lethality, but there may also be a secondary mutation in the MD701 strain that further increases embryonic lethality.

Comments

microPublication Biology, 2025. DOI.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Recommended Citation

Zampetti, Nina M.; Quaglia, Kristin A.; and Petrella, Lisa N., "The lim-7p::ced-1::GFP Transgene From the MD701 Strain Increases Embryonic Lethality in c. elegans" (2025). Biological Sciences Faculty Research and Publications. 1042.
https://epublications.marquette.edu/bio_fac/1042