Perturbing Microtubule Integrity Blocks AMP-Activated Protein Kinase-Induced Meiotic Resumption in Cultured Mouse Oocytes

Authors

Ru Ya, Marquette University
Stephen Downs, Marquette UniversityFollow

Document Type

Article

Language

eng

Format of Original

12 p.

Publication Date

2012

Publisher

Cambridge University Press

Source Publication

Zygote

Source ISSN

1469-8730

Abstract

The oocyte meiotic spindle is comprised of microtubules (MT) that bind chromatin and regulate both metaphase plate formation and karyokinesis during meiotic maturation; however, little information is known about their role in meiosis reinitiation. This study was conducted to determine if microtubule integrity is required for meiotic induction and to ascertain how it affects activation of AMP-activated protein kinase (AMPK), an important participant in the meiotic induction process. Treatment with microtubule-disrupting agents nocodazole and vinblastine suppressed meiotic resumption in a dose-dependent manner in both arrested cumulus cell-enclosed oocytes (CEO) stimulated with follicle-stimulating hormone (FSH) and arrested denuded oocytes (DO) stimulated with the AMPK activator, 5-aminoimidazole-4-carboxamide-1-beta-4-ribofuranoside (AICAR). This effect coincided with suppression of AMPK activation as determined by western blotting and germinal vesicle immunostaining. Treatment with the MT stabilizer paclitaxel also suppressed meiotic induction. Targeting actin filament polymerization had only a marginal effect on meiotic induction. Immunolocalization experiments revealed that active I and II stages, while it localized at the spindle midzone during anaphase. This discrete localization pattern was dependent on MT integrity. Treatment with nocodazole led to disruption of proper spindle pole localization of active AMPK, while paclitaxel induced excessive polymerization of spindle MT and formation of ectopic asters with accentuated AMPK colocalization. Although stimulation of AMPK increased the rate of germinal vesicle breakdown (GVB), spindle formation and polar body (PB) extrusion, the kinase had no effect on peripheral movement of the spindle. These data suggest that themeiosis-inducing action and localization of AMPK are regulated byMT spindle integrity during mouse oocyte maturation.

Comments

Published version. Zygote (2012): 1-12. DOI. © 2012 Cambridge University Press. Used with permission.

Recommended Citation

Ya, Ru and Downs, Stephen, "Perturbing Microtubule Integrity Blocks AMP-Activated Protein Kinase-Induced Meiotic Resumption in Cultured Mouse Oocytes" (2012). Biological Sciences Faculty Research and Publications. 115.
https://epublications.marquette.edu/bio_fac/115