Transplants of Embryonic Brainstem Containing the Locus Coeruleus into Spinal Cord Enhance the Hindlimb Flexion Reflex in Adult Rats

Document Type

Article

Language

eng

Format of Original

12 p.

Publication Date

9-3-1986

Publisher

Elsevier

Source Publication

Brain Research

Source ISSN

0006-8993

Abstract

Cell suspensions of embryonic brainstem containing the locus coeruleus were injected intervertebrally into the lumbar spinal cord of adult rats whose descending catecholamine (CA) fibers had been lesioned with intracisternal injection of 6-hydroxydopamine. Up to 1100 CA cells were found 2 and 4 months later, and these cells grew processes which produced histologically detectable reinnervation of the lumbar gray matter on the injected side of the cord. To assess the functional activity of the transplanted CA cells, the force of the hindlimb flexion reflex was measured in acute spinal rats. This reflex has been shown previously to be strongly enhanced by catecholamines. The flexion reflexes were significantly stronger in the transplanted rats than in the controls. Further, the flexion reflexes were significantly reduced by phenoxybenzamine, an α-adrenergic blocker, in the transplanted rats while the reflexes of controls were not significantly changed. These results demonstrate that cell suspension transplants of embryonic brainstem containing the locus coeruleus into the adult rat spinal cord survive, grow reinnervating catecholamine processes, and can affect the functional activity of the spinal cord.

Comments

Brain Research, Vol. 381, No. 2 (September 3, 1986): 225-236. DOI.

James Buchanan was affiliated with the Colorado State University - Fort Collins at the time of publication.

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