Document Type
Article
Language
eng
Format of Original
19 p.
Publication Date
10-1986
Publisher
Oxford University Press
Source Publication
Nucleic Acids Research
Source ISSN
0305-1048
Original Item ID
doi: 10.1093/nar/14.20.8007
Abstract
A small family of DNA sequences Is rearranged during the development of the somatic nucleus in Tetrahymena. The family is defined by 266 bp of highly conserved sequence which restriction mapping, hybridization and sequence analysis have shown is shared by a cloned micronuclear fragment and three sequences which constitute the macronuclear family. Genomic Southern hybridization experiments indicate there are five members of the family in micronuclear DNA. All of the family members are present in whole genome homozygotes and are therefore nonallellic. The three macronuclear sequences are all present in clonal cell lines and are reproducibly generated in every developing macronucleus. The rearrangement event begins 14 hours after conjugation is initiated and is nearly completed by 16 hours.
Recommended Citation
Allitto, Bernice A. and Karrer, Kathleen M., "A Family of DNA Sequences is Reproducibly Rearranged in the Somatic Nucleus of Tetrahymena" (1986). Biological Sciences Faculty Research and Publications. 238.
https://epublications.marquette.edu/bio_fac/238
Comments
Published version. Nucleic Acids Research, Vol. 14, No. 20 (October 1986): 8007-8025. DOI. © 1986 Oxford University Press. Used with permission.
Kathleen Karrer was affiliated with Brandeis University at the time of publication.