Document Type




Format of Original

19 p.

Publication Date



Oxford University Press

Source Publication

Nucleic Acids Research

Source ISSN


Original Item ID

doi: 10.1093/nar/14.20.8007


A small family of DNA sequences Is rearranged during the development of the somatic nucleus in Tetrahymena. The family is defined by 266 bp of highly conserved sequence which restriction mapping, hybridization and sequence analysis have shown is shared by a cloned micronuclear fragment and three sequences which constitute the macronuclear family. Genomic Southern hybridization experiments indicate there are five members of the family in micronuclear DNA. All of the family members are present in whole genome homozygotes and are therefore nonallellic. The three macronuclear sequences are all present in clonal cell lines and are reproducibly generated in every developing macronucleus. The rearrangement event begins 14 hours after conjugation is initiated and is nearly completed by 16 hours.


Published version. Nucleic Acids Research, Vol. 14, No. 20 (October 1986): 8007-8025. DOI. © 1986 Oxford University Press. Used with permission.

Kathleen Karrer was affiliated with Brandeis University at the time of publication.

Included in

Biology Commons