Nuclear RNA Surveillance in Saccharomyces cerevisiae: Trf4p-dependent Polyadenylation of Nascent Hypomethylated tRNA and an Aberrant Form of 5S rRNA

Authors

Sujatha A. Kadaba, Marquette University
Xuying Wang, Marquette UniversityFollow
James T. Anderson, Marquette UniversityFollow

Document Type

Article

Language

eng

Format of Original

14 p.

Publication Date

3-2006

Publisher

Cold Spring Harbor Laboratory Press

Source Publication

RNA

Source ISSN

1355-8382

Original Item ID

doi: 10.1261/rna.2305406; PubMed Central, PMCID: PMC1383588

Abstract

1-Methyladenosine modification at position 58 of tRNA is catalyzed by a two-subunit methyltransferase composed of Trm6p and Trm61p in Saccharomyces cerevisiae. Initiator tRNA (tRNA_i^Met) lacking m¹A58 (hypomethylated) is rendered unstable through the cooperative function of the poly(A) polymerases, Trf4p/Trf5p, and the nuclear exosome. We provide evidence that a catalytically active Trf4p poly(A) polymerase is required for polyadenylation of hypomethylated tRNA_i^Met in vivo. DNA sequence analysis of tRNA_i^Met cDNAs and Northern hybridizations of poly(A)+ RNA provide evidence that nascent pre-tRNA_i^Met transcripts are targeted for polyadenylation and degradation. We determined that a mutant U6 snRNA and an aberrant form of 5S rRNA are stabilized in the absence of Trf4p, supporting that Trf4p facilitated RNA surveillance is a global process that stretches beyond hypomethylated tRNA_i^Met. We conclude that an array of RNA polymerase III transcripts are targeted for Trf4p/ Trf5p-dependent polyadenylation and turnover to eliminate mutant and variant forms of normally stable RNAs.

Comments

Published version. RNA, Vol. 12, No. 3 (March 2006): 508-521. DOI. © 2006 Cold Spring Harbor Laboratory Press. Used with permission.

Recommended Citation

Kadaba, Sujatha A.; Wang, Xuying; and Anderson, James T., "Nuclear RNA Surveillance in Saccharomyces cerevisiae: Trf4p-dependent Polyadenylation of Nascent Hypomethylated tRNA and an Aberrant Form of 5S rRNA" (2006). Biological Sciences Faculty Research and Publications. 323.
https://epublications.marquette.edu/bio_fac/323