Synthesis of Normal and “Immunogenic RNA” in Peritoneal Macrophage Cells
Document Type
Article
Language
eng
Format of Original
9 p.
Publication Date
10-1967
Publisher
American Association of Immunologists
Source Publication
The Journal of Immunology
Source ISSN
0022-1767
Abstract
RNA extracted from rat peritoneal macrophage cells following the phagocytosis of sheep red blood cells (SRBC) caused the synthesis of specific hemagglutinating antibody by normal rat spleen cells. The majority of the biological activity of the RNA was contained in the lightest of three distinct regions, the 6 to 10 S region identified by sucrose density ultracentrifugation. Pulse-labeling studies indicate that synthesis of RNA in the 6 to 10 S region is completed 30 min following the pulse in peritoneal cells during the phagocytosis of SRBC, and 60 min following the pulse in cells not exposed to antigen. Pulse-chase studies show that this RNA remains stable for at least 2 ½ hr following the pulse. Both the antibody response, and the synthesis of RNA in the 6 to 10 S region were inhibited by actinomycin D.
Recommended Citation
Bishop, David Clarence; Pisciotta, Anthony V.; and Abramoff, Peter, "Synthesis of Normal and “Immunogenic RNA” in Peritoneal Macrophage Cells" (1967). Biological Sciences Faculty Research and Publications. 350.
https://epublications.marquette.edu/bio_fac/350
Comments
The Journal of Immunology, Vol. 99, No. 4 (October 1967): 751-759. DOI.