Cosmid Cloning of Five Zymomonas trp Genes by Complementation of Escherichia coli and Pseudomonas putida trp Mutants
Format of Original
American Society for Microbiology
Journal of Bacteriology
Original Item ID
A library of Zymomonas mobilis genomic DNA was constructed in the broad-host-range cosmid pLAFR1. The library was mobilized into a variety of Escherichia coli and Pseudomonas putida trp mutants by using the helper plasmid pRK2013. Five Z. mobilis trp genes were identified by the ability to complement the trp mutants. The trpF, trpB, and trpA genes were on one cosmid, while the trpD and trpC genes were on two separate cosmids. The organization of the Z. mobilis trp genes seems to be similar to the organization found in Rhizobium spp., Acinetobacter calcoaceticus, and Pseudomonas acidovorans. The trpF, trpB, and trpA genes appeared to be linked, but they were not closely associated with trpD or trpC genes.
Eddy, Christina Kay; Smith, Oliver H.; and Noel, K. Dale, "Cosmid Cloning of Five Zymomonas trp Genes by Complementation of Escherichia coli and Pseudomonas putida trp Mutants" (1988). Biological Sciences Faculty Research and Publications. 400.