Document Type
Article
Language
eng
Format of Original
7 p.
Publication Date
4-2015
Publisher
Oxford University Press
Source Publication
FEMS Microbiology Letters
Source ISSN
0378-1097
Original Item ID
DOI: 10.1093/femsle/fnv029
Abstract
Among the few bacteria known to utilize methyl tert-butyl ether (MTBE) as a sole carbon source, Methylibium petroleiphilum PM1 is a well-characterized organism with a sequenced genome; however, knowledge of the genetic regulation of its MTBE degradation pathway is limited. We investigated the role of a putative transcriptional activator gene, mdpC, in the induction of MTBE-degradation genes mdpA (encoding MTBE monooxygenase) and mdpJ (encoding tert-butyl alcohol hydroxylase) of strain PM1 in a gene-knockout mutant mdpC−. We also utilized quantitative reverse transcriptase PCR assays targeting genes mdpA, mdpJ and mdpC to determine the effects of the mutation on transcription of these genes. Our results indicate that gene mdpC is involved in the induction of both mdpA and mdpJ in response to MTBE and tert-butyl alcohol (TBA) exposure in PM1. An additional independent mechanism may be involved in the induction of mdpJ in the presence of TBA.
Recommended Citation
Joshi, Geetika; Schmidt, Radomir; Scow, Kate M.; Denison, Michael S.; and Hristova, Krassimira R., "Gene mdpC Plays a Regulatory Role in the Methyl-tert-butyl Ether Degradation Pathway of Methylibium petroleiphilum Strain PM1" (2015). Biological Sciences Faculty Research and Publications. 407.
https://epublications.marquette.edu/bio_fac/407
Comments
Accepted version. FEMS Microbiology Letters, Vol. 362, No. 7 (April 2015). DOI. © 2015 FEMS. Used with permission.