Document Type
Article
Language
eng
Format of Original
10 p.
Publication Date
8-2013
Publisher
Nature Publishing Group
Source Publication
Nature Communications
Source ISSN
2041-1723
Original Item ID
doi: 10.1038/ncomms3281
Abstract
Srs2 dismantles presynaptic Rad51 filaments and prevents its re-formation as an anti-recombinase. However, the molecular mechanism by which Srs2 accomplishes these tasks remains unclear. Here we report a single-molecule fluorescence study of the dynamics of Rad51 filament formation and its disruption by Srs2. Rad51 forms filaments on single-stranded DNA by sequential binding of primarily monomers and dimers in a 5′–3′ direction. One Rad51 molecule binds to three nucleotides, and six monomers are required to achieve a stable nucleation cluster. Srs2 exhibits ATP-dependent repetitive motion on single-stranded DNA and this activity prevents re-formation of the Rad51 filament. The same activity of Srs2 cannot prevent RecA filament formation, indicating its specificity for Rad51. Srs2’s DNA-unwinding activity is greatly suppressed when Rad51 filaments form on duplex DNA. Taken together, our results reveal an exquisite and highly specific mechanism by which Srs2 regulates the Rad51 filament formation.
Recommended Citation
Qiu, Yupeng; Antony, Edwin; Doganay, Sultan; Koh, Hye Ran; Lohman, Timothy M.; and Myong, Sua, "Srs2 Prevents Rad51 Filament Formation by Repetitive Motion on DNA" (2013). Biological Sciences Faculty Research and Publications. 428.
https://epublications.marquette.edu/bio_fac/428
Comments
Published version. Nature Communications, Vol. 4 (August 2013). DOI. © 2013 Nature Publishing Group. Used with permission.
Edwin Antony was affiliated with Utah State University at the time of publication.