Phosphorylated β-guanidinopropionate as a Substitute for Phosphocreatine in Rat Muscle
Document Type
Article
Language
eng
Publication Date
4-1975
Publisher
American Physiological Society
Source Publication
American Journal of Physiology
Source ISSN
0002-9513
Original Item ID
DOI: 10.1152/ajplegacy.1975.228.4.1123
Abstract
To evaluate phosphorylated β-guanidinopropionate (β-GPAP) as a substitute for phosphocreatine (PC), hypoxic tibialis anterior muscles were stimulated to contract isometrically in situ until twitch tension fell to 25% of the peak value. Muscles from rats fed β-guanidinopropionic acid (β-GPA) failed to exhibit the staircase phenomenon, and they developed 28% less tension than control muscles. In control muscles lactate increased from 0.75 to 20.99, ADP increased from 0.89 to 1.20, ATP decreased from 5.09 to 2.73, and PC decreased from 15.78 to 1.52 μmol/g. In muscles from rats fed β-GPA, lactate increased from 0.85 to 14.31, ADP increased from 0.86 to 1.06, ATP decreased from 2.69 to 1.71, PC decreased from 0.73 to 0.30, and β-GPAP decreased from 30.34 to 19.45 μmol/g. From these measurements, the use of high-energy phosphate was calculated to be reduced 32% in muscles from rats fed β-GPA. The relationships between the use of high-energy phosphate and tension development confirm experimentally the ability of β-GPAP to substitute for PC as a source of energy to sustain muscle contraction.
Recommended Citation
Fitch, Coy D.; Jellinek, Max; Fitts, Robert H.; Baldwin, K. M.; and Holloszy, J. O., "Phosphorylated β-guanidinopropionate as a Substitute for Phosphocreatine in Rat Muscle" (1975). Biological Sciences Faculty Research and Publications. 446.
https://epublications.marquette.edu/bio_fac/446
Comments
American Journal of Physiology, Vol. 228, No. 4 (April 1975): 1123-1125. DOI.
Robert Fitts was affiliated with Washington University at the time of publication.