Effect of Thyrotoxicosis on Sarcoplasmic Reticulum in Rat Skeletal Muscle

Document Type

Article

Language

eng

Format of Original

5 p.

Publication Date

9-1982

Publisher

American Physiological Society

Source Publication

American Journal of Physiology - Cell Physiology

Source ISSN

0002-9513

Original Item ID

DOI: 10.1152/ajpcell.1982.243.3.C151

Abstract

The effect of thyrotoxicosis on the capacity of fragmented sarcoplasmic reticulum (FSR) and crude homogenate (CH) to sequester Ca2+ was determined in rat muscle for the slow-twitch type I soleus (SOL), the fast-twitch type IIA deep region of the vastus lateralis (DVL),and the fast-twitch type IIB superficial region of the vastus lateralis (SVL). The maximal rate of Ca2+ uptake (Vmax) and Km were determined in both the CH and FSR preparations, and the total Ca2+ uptake capacity of the CH was determined. In the slow SOL, thyrotoxicosis increased the Vmax (8.20 ± 0.96 vs. 15.70 ± 0.92 µmol Ca2+ · g wet muscle-1 · min-1) and the total Ca2+uptake (17.62 ± 1.30 vs. 27.13 ± 2.16 µmol Ca2+ · g wet muscle-1) of the CH preparation. Thyrotoxicosis increased the FSR yield 2.3-fold in the slow-twitch SOL; however, the kinetic characteristics (Vmax and Km) of these vesicles were not altered. Thyrotoxicosis had no effect on the CH and FSR preparations in either the type IIA or type IIB sample. These results can be explained by a thyroid hormone-mediated increase in the quantity of the sarcoplasmic reticulum in type I muscle and suggest no effect on the hormone on the qualitative nature of the Ca2+-enzyme interaction.

Comments

American Journal of Physiology - Cell Physiology, Vol. 243, No. 3 (September 1982): C151-C155. DOI.

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