Document Type

Article

Language

eng

Publication Date

9-1997

Publisher

American Phytopathological Society

Source Publication

Molecular Plant-Microbe Interactions

Source ISSN

0894-0282

Abstract

Exudates released from germinating seeds and roots of a black-seeded bean (Phaseolus vulgaris cv. Midnight Black Turtle Soup) induce an antigenic change in the lipopolysaccharide (LPS) of Rhizobium etli CE3. By spectroscopic analyses and chromatographic comparisons with derived standards, the chemical structures of the aglycone portions of the major inducing molecules from seed exudate were deduced, and they were identified as delphinidin, cyanidin, petunidin, and malvidin. These anthocyanidins were present in seed exudate mainly as glycosides, the chief inducer being delphinidin 3-glucoside. Also present were 3-glucosides of petunidin and malvidin and glycosides of cyanidin and delphinidin. Seed exudate from a bean variety deficient in anthocyanins did not induce the LPS conversion. The ability of root exudate to induce an antigenic change in the LPS was due to compounds other than anthocyanins.

Comments

Published version. Molecular Plant-Microbe Interactions, Vol. 10, No. 7 (September 1997): 903-910. DOI. © 1997 American Phytopathological Society. Used with permission.

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