Title

Isolation of Auxotrophs and Analysis of Regulation of Tryptophan Biosynthesis in Zymomonas mobilis

Document Type

Article

Language

eng

Publication Date

4-1988

Publisher

Springer

Source Publication

Archives of Microbiology

Source ISSN

0302-8933

Abstract

Tryptophan auxotrophs were isolated and used to analyze the regulation of tryptophan biosynthesis in Zymomonas mobilis. Twelve tryptophan auxotrophs were cassified as trp E, Bor A based on accumulation of, or growth on, indole and anthranilic acid. Trp B mutants were found to accumulate indole when grown on limiting, but not on excess tryptophan, suggesting that tryptophan plays a role in regulating its biosynthesis. Tryptophan synthase and indoleglycerol phosphate synthase specific activities were measured in the wild-type strain and two trp mutants grown in limiting or excess tryptophan. Neither activity was repressed by exogenous tryptophan.

Comments

Archives of Microbiology, Vol. 149, No. 6 (April 1988): 561-564. DOI.

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