A Genetic Tool to Track Protein Aggregates and Control Prion Inheritance
Document Type
Article
Language
Eng
Publication Date
11-2-2017
Publisher
Elsevier (Cell Press)
Source Publication
Cell
Source ISSN
0092-8674
Abstract
Protein aggregation is a hallmark of many diseases but also underlies a wide range of positive cellular functions. This phenomenon has been difficult to study because of a lack of quantitative and high-throughput cellular tools. Here, we develop a synthetic genetic tool to sense and control protein aggregation. We apply the technology to yeast prions, developing sensors to track their aggregation states and employing prion fusions to encode synthetic memories in yeast cells. Utilizing high-throughput screens, we identify prion-curing mutants and engineer “anti-prion drives” that reverse the non-Mendelian inheritance pattern of prions and eliminate them from yeast populations. We extend our technology to yeast RNA-binding proteins (RBPs) by tracking their propensity to aggregate, searching for co-occurring aggregates, and uncovering a group of coalescing RBPs through screens enabled by our platform. Our work establishes a quantitative, high-throughput, and generalizable technology to study and control diverse protein aggregation processes in cells.
Recommended Citation
Newby, Gregory A.; Kiriakov, Szilvia; Hallacli, Erinc; Kayatekin, Can; Tsvetkov, Peter; Mancuso, Christopher P.; Bonner, J. Maeve; Hesse, William R.; Chakrabortee, Sohini; Manogaran, Anita L.; Liebman, Susan W.; Lindquist, Susan; and Khalil, Ahmad S., "A Genetic Tool to Track Protein Aggregates and Control Prion Inheritance" (2017). Biological Sciences Faculty Research and Publications. 612.
https://epublications.marquette.edu/bio_fac/612
Comments
Cell, Vol. 171, No. 4 (November 2, 2017): 966-979. DOI.