Document Type
Article
Language
eng
Publication Date
3-28-2017
Publisher
Cold Spring Harbor Laboratory Press
Source Publication
RNA: A Publication of the RNA Society
Source ISSN
1355-8382
Abstract
RNA surveillance via the nuclear exosome requires cofactors such as the helicase SKIV2L2 to process and degrade certain noncoding RNAs. This research aimed to characterize the phenotype associated with RNAi knockdown of Skiv2l2 in two murine cancer cell lines: Neuro2A and P19. SKIV2L2 depletion in Neuro2A and P19 cells induced changes in gene expression indicative of cell differentiation and reduced cellular proliferation by 30%. Propidium iodide-based cell-cycle analysis of Skiv2l2 knockdown cells revealed defective progression through the G2/M phase and an accumulation of mitotic cells, suggesting SKIV2L2 contributes to mitotic progression. Since SKIV2L2 targets RNAs to the nuclear exosome for processing and degradation, we identified RNA targets elevated in cells depleted of SKIV2L2 that could account for the observed twofold increase in mitotic cells. Skiv2l2knockdown cells accumulated replication-dependent histone mRNAs, among other RNAs, that could impede mitotic progression and indirectly trigger differentiation.
Recommended Citation
Onderak, Alexis Marie and Anderson, James T., "Loss of The RNA Helicase SKIV2L2 Impairs Mitotic Progression and Replication-Dependent Histone mRNA Turnover in Murine Cell Lines" (2017). Biological Sciences Faculty Research and Publications. 618.
https://epublications.marquette.edu/bio_fac/618
Comments
Published version. RNA, Vol. 23 (2017): 910-926. DOI. © 2017 Onderak and Anderson; Published by Cold Spring Harbor Laboratory Press for the RNA Society. Used with permission.