Document Type

Article

Language

eng

Format of Original

9 p.

Publication Date

1-2003

Publisher

American Phytopathological Society

Source Publication

Molecular Plant-Microbe Interactions

Source ISSN

0894-0282

Original Item ID

doi: 10.1094/MPMI.2003.16.1.65

Abstract

Rhizobium etli CFN42 bacteroids from bean nodules possessed an abundant 16-kDa protein (BacS) that was found in the membrane pellet after cell disruption. This protein was not detected in bacteria cultured in tryptone-yeast extract. In minimal media, it was produced at low oxygen concentration but not in a mutant whose nifA was disrupted. N-terminal sequencing of the protein led to isolation of a bacS DNA fragment. DNA hybridization and nucleotide sequencing revealed three copies of the bacS gene, all residing on the main symbiotic plasmid of strain CFN42. A stretch of 304 nucleotides, exactly conserved upstream of all three bacS open reading frames, had very close matches with the NifA and sigma 54 consensus binding sequences. The only bacS homology in the genetic sequence databases was to three hypothetical proteins of unknown function, all from rhizobial species. Mutation and genetic complementation indicated that each of the bacS genes gives rise to a BacS polypeptide. Mutants disrupted or deleted in all three genes did not produce the BacS polypeptide but were Nod+ and Fix+ on Phaseolus vulgaris.

Comments

Published version. Molecular Plant-Microbe Interactions, Vol. 16, No. 1 (January 2003): 65-73. DOI. © 2003 American Phytopathological Society. Used with permission.

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