Activity of Methanogenic Biomass After Heat and Freeze Drying in Air

Authors

Ujwal H. Bhattad, Marquette University
Kaushik Venkiteshwaran, Marquette UniversityFollow
Keerthi Cherukuri, Marquette University
James Maki, Marquette UniversityFollow
Daniel Zitomer, Marquette UniversityFollow

Document Type

Article

Language

eng

Publication Date

4-2017

Publisher

Royal Society of Chemistry

Source Publication

Environmental Science: Water Research and Technology

Source ISSN

2053-1419

Abstract

It would be beneficial if methanogenic cultures could be preserved for anaerobic digester bioaugmentation or as seed for standard tests such as biochemical methane potential. However, storage of wet culture or drying in anaerobic atmosphere may not be economically feasible. In this study, the effect of heat and freeze drying in ambient air on the methanogenic activity of an anaerobic culture was determined. The anaerobic culture was dried in air at 104 °C for 12 h, and by freezing at −196 °C in air with subsequent drying at subzero temperatures. The rehydrated culture consistently produced CH₄ from H₂:CO₂ and acetate after drying. Drying caused a greater decrease in acetate methanogenic activity compared to H₂:CO₂ methanogenic activity. Transcript qPCR results for a functional gene in methanogens (mcrA) also revealed significant survivability of rehydrated methanogenic populations. Inactivation due to drying differed among genera, with least to most inactivation in the order Methanospirillum < Methanosaeta < Methanoculleus.

Comments

Environmental Science: Water Research and Technology, Vol. 3 (2017): 462-471. DOI.

Recommended Citation

Bhattad, Ujwal H.; Venkiteshwaran, Kaushik; Cherukuri, Keerthi; Maki, James; and Zitomer, Daniel, "Activity of Methanogenic Biomass After Heat and Freeze Drying in Air" (2017). Biological Sciences Faculty Research and Publications. 641.
https://epublications.marquette.edu/bio_fac/641