Document Type

Article

Language

eng

Publication Date

2009

Publisher

Japan Society of Smooth Muscle Research

Source Publication

Journal of Smooth Muscle Research

Source ISSN

0916-8737

Abstract

This study was designed to test the hypothesis that there is heterogeneous expression of contractile and structural proteins between the smooth muscle cells (SMCs) in the inner and outer circular muscle (ICM and OCM) layers of the ileum. Immunohistochemical staining and quantitation of fresh frozen sections of the dog ileum was performed using protein specific antibodies. Smooth muscle (SM) SMA myosin heavy chain (MHC), α- and γ-SM actin, and vinculin all show greater expression in the ICM relative to the OCM. SMB MHC and fibronectin show the opposite pattern, with greater expression in the OCM relative to the ICM. Differences in expression of these proteins are consistent with proposed differences in function of these muscle layers. Hypotheses regarding muscle tone and the coordination and regulation of peristalsis via these different muscle layers based on this data can now be made and tested.

Comments

Published version. Journal of Smooth Muscle Research, Vol. 45, No. 5 (2009): 217-230. DOI. © 2009 Japan Society of Smooth Muscle Research. Used with permission.

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