Document Type
Article
Language
eng
Publication Date
12-2016
Publisher
Elsevier
Source Publication
Surgery for Obesity and Related Diseases
Source ISSN
1550-7289
Abstract
Background
Prader-Willi syndrom (PWS) is a genetic disorder characterized by hyperphagia, obesity, cardiopulmonary diseases, and increased mortality. Although successful weight loss improves health in PWS, few treatments cause sustained weight loss in obese patients let alone obese individuals with PWS.
Objectives
The present study uses the Magel2 knockout (KO) mouse, an animal model of PWS, to conduct a preclinical study on the efficacy of sleeve gastrectomy(SG) in PWS.
Setting
Academic research laboratory, United States.
Methods
We performed sham or SG surgeries in 24- to 28-week-old male Magel2 KO and wild-type littermate control mice (WT) who had been maintained on a high-fat diet for 10 weeks. We monitored weight, food intake, and fat and lean mass pre- and postoperatively. Fasting glucose, glucose tolerance, and counter-regulation were measured postoperatively.
Results
Magel2 KO animals had similar recovery and mortality rates compared with WT. SG resulted in similar weight loss, specifically loss of fat but not lean mass, in both Magel2 KO and WT mice. SG also resulted in significantly lower fasting glucose levels and a reduction in fat intake in both Magel2 KO and WT mice. We also found that Magel2 KO mice failed to increase their food intake in response to the glucoprivic agent 2-deoxy-D-glucose, suggesting impaired glucose counter-regulation, but this occurred regardless of surgical status. All results were considered significant when P< .05.
Conclusion
We find in this mouse model of PWS, SG is a well-tolerated, effective strategy for weight and fat loss.
Recommended Citation
Arble, Deanna M.; Pressler, Joshua W.; Sorrell, Joyce; Wevrick, Rachel; and Sandoval, Darleen A., "Sleeve Gastrectomy Leads to Weight Loss in the Magel2 Knockout Mouse" (2016). Biological Sciences Faculty Research and Publications. 655.
https://epublications.marquette.edu/bio_fac/655
Comments
Accepted version. Surgery for Obesity and Related Diseases, Vol. 12, No. 10 (December 2016): 1795-1802. DOI. © 2016 Elsevier B.V. Used with permission.
Deanna M. Arble was affiliated with the University of Michigan, Ann Arbor at the time of publication.