Document Type
Article
Publication Date
8-2020
Publisher
Company of Biologists
Source Publication
Journal of Cell Science
Source ISSN
0021-9533
Abstract
The radial spoke is a key element in a transducer apparatus controlling the motility of eukaryotic cilia. The transduction biomechanics is a long-standing question in cilia biology. The radial spoke has three regions – a spoke head, a bifurcated neck and a stalk. Although the neck and the stalk are asymmetric, twofold symmetry of the head has remained controversial. In this work we used single particle cryo-electron microscopy (cryo-EM) analysis to generate a 3D structure of the whole radial spoke at unprecedented resolution. We show the head region at 15 Å (1.5 nm) resolution and confirm twofold symmetry. Using distance constraints generated by cross-linking mass spectrometry, we locate two components, RSP2 and RSP4, at the head and neck regions. Our biophysical analysis of isolated RSP4, RSP9, and RSP10 affirmed their oligomeric state. Our results enable us to redefine the boundaries of the regions and propose a model of organization of the radial spoke component proteins.
Recommended Citation
Poghosyan, Emiliya; Iacovache, Ioan; Faltova, Lenka; Leitner, Alexander; Yang, Pinfen; Diener, Dennis R.; Aebersold, Ruedi; Zuber, Benoit; and Ishikawa, Takashi, "The Structure and Symmetry of the Radial Spoke Protein Complex in Chlamydomonas Flagella" (2020). Biological Sciences Faculty Research and Publications. 842.
https://epublications.marquette.edu/bio_fac/842
Comments
Published version. Journal of Cell Science, Vol. 133, No. 16 (August 2020): jcs245233. DOI. © 2020 Company of Biologists. Used with permission.